A New Positive/Negative Selection Scheme for Precise BAC Recombineering
نویسندگان
چکیده
منابع مشابه
Simple and highly efficient BAC recombineering using galK selection
Recombineering allows DNA cloned in Escherichia coli to be modified via lambda (lambda) Red-mediated homologous recombination, obviating the need for restriction enzymes and DNA ligases to modify DNA. Here, we describe the construction of three new recombineering strains (SW102, SW105 and SW106) that allow bacterial artificial chromosomes (BACs) to be modified using galK positive/negative selec...
متن کاملComparing selection schemes in BAC - recombineering method of tagging a novel Drosophila gene
Sialylation plays an important role in the Drosophila nervous system. However, the regulation of sialylation pathway remains poorly understood. We focused our analysis on a novel gene, CMP-Sialic acid synthetase (CSAS), that is predicted to be a key player of the pathway. In order to investigate its expression, we decided to introduce a tag sequence into the CSAS genomic locus within a BAC clon...
متن کاملRecombineering of BAC DNA for the Generation of Transgenic Mice
The use of BAC plasmids for transgenic gene expression is also gaining popularity over traditional proximal promoter driven transgene expression because the BAC typically contain most, if not all, of the important regulatory elements required to recapitulate endogenous gene expression (Giraldo and Montoliu 2001). Maintaining the coding sequence within its regulatory locus insulates the transgen...
متن کاملEfficient conditional knockout targeting vector construction using co-selection BAC recombineering (CoSBR)
A simple and efficient strategy for Bacterial Artificial Chromosome (BAC) recombineering based on co-selection is described. We show that it is possible to efficiently modify two positions of a BAC simultaneously by co-transformation of a single-stranded DNA oligo and a double-stranded selection cassette. The use of co-selection BAC recombineering reduces the DNA manipulation needed to make a c...
متن کاملA new approach for sustainable supplier selection
Recently, sustainable supply chain management (SSCM) has become one of the important subjects in the industry and academia. Supplier selection, as a strategic decision, plays a significant role in SSCM. Researchers use different multi-criteria decision making (MCDM) methods to evaluate and select sustainable suppliers. In the previous studies, evaluation is solely based on the desirable feature...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular Biotechnology
سال: 2009
ISSN: 1073-6085,1559-0305
DOI: 10.1007/s12033-009-9142-3